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Plasmid DNAs interactions with lanthanoide compounds
Budko, Kateryna ; Horák, Daniel (referee) ; Rittich, Bohuslav (advisor)
Recently much attention is given to lanthanides and their complexes as excellent catalysts for cleavage of nucleic acids. The thesis has been focused on the cleavage of plasmid and bacterial DNA by ions Nd3+ and Y3+ and by different carriers containing the lanthanide compounds. The creation of single-stranded nicks and double-stranded ones in the plasmid DNA molecules was studied by agarose gel electrophoresis. Verification of the cleavage of bacterial DNA was made by polymerase chain reaction using primers specific for the domain Bacteria and genus and species-specific primers. The results will be used in the development of the method that will allow perfect carriers`s coverage verification with the magnetic perovskit nucleus and other carriers with the lanthanide compounds.
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Šumava National Park and Interests of its Municipalities: The Case of Postmaterial Cleavages?
Musilová, Karolína ; Jüptner, Petr (advisor) ; Švec, Kamil (referee)
The diploma thesis Šumava National Park and Interests of its Municipalities: The Case of Postmaterial Cleavages? analyses, if the postmaterial cleavage is present in the case of Šumava National Park and how is it projected on the national level. Since the very beginning, there have been argues about the notion of the national park, where two different set of ideas collide. On the one hand, there are local municipalities which depend on incomes from tourism, on the other hand, the environmentalists seek for growing extent of areas without any human intervention. The theoretical background is based on concept of cleavages by S. Rokkan and S.M. Lipset. Special attention is paid to the postmaterial dimension of the cleavage, which was evolved by R. Inglehart. The analytical part consists of case study, which examines the attitudes of the stakeholders. The examination is based on semi-structured interviews with mayors, official documents and media coverage of the topic. According to the findings, there is a cleavage, which is visible on all levels of political system, including political parties and presidents.
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Electoral Geography of Poland After 1989
Šulc, Jan ; Mlejnek, Josef (advisor) ; Kubát, Michal (referee)
The master's thesis "Electoral Geography of Poland After 1989" explores the spatial distribution of patterns of electoral behavior in Poland on the basis of analysis of the results of all presidential elections and elections to the Sejm that took place in the country between the years 1990-2010. The main hypothesis tested by this master thesis is that former boundaries between Russia, Prussia (Germany) and Austria (Austria-Hungary), between which the territory of modern-day Poland was divided in the 19th century, still have an impact on Poland's electoral geography. On the basis of this hypothesis, political parties and presidential candidates are divided into two pre-defined categories: traditionalist and modernist The validity of the hypothesis is then proven by visual analysis of political parties' and presidential candidates' electoral maps, and also by the application of Pearson's correlation coefficient on political parties' electoral results from constituencies and presidential candidates' electoral results from voivodships. In contemporary Poland, a clear rift exists between the parts of the country formerly dominated by Prussia (Germany) and major Polish cities on the one hand (also known as "Poland A"), and Russia and Austria (Austria-Hungary) on the other hand (which is sometimes...
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Electoral Geography of Poland After 1989
Šulc, Jan ; Mlejnek, Josef (advisor) ; Kubát, Michal (referee)
The rigorous thesis Electoral Geography of Poland After 1989. The Spatial Dimension of the Results of Presidential Elections and Elections to the Sejm of the Republic of Poland explores the spatial distribution of patterns of electoral behavior in Poland on the basis of analysis of the results of all presidential elections and elections to the Sejm that took place in the country between the years 1990-2011. The main hypothesis tested in this rigorous thesis is that former boundaries between Russia, Prussia (Germany) and Austria (Austria-Hungary), between which the territory of modern-day Poland was divided in the 19th century, still have an impact on Poland's electoral geography. On the basis of this hypothesis political parties and presidential candidates are divided into two pre-defined categories: traditionalist and modernist. The validity of the hypothesis is then proven by visual analysis of political parties' and presidential candidates' electoral maps, and also by the application of Pearson's correlation coefficient on political parties' electoral results from constituencies and presidential candidates' electoral results from voivodships. In contemporary Poland a clear rift exists between the parts of the country formerly dominated by Prussia (Germany) and major Polish cities on the one hand (also...
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Splitting and Dissociation in Schizophrenia
Pěč, Ondřej ; Raboch, Jiří (advisor) ; Šípek, Jiří (referee) ; Kocourková, Jana (referee)
The term splitting is defined as a process of formation of mental aggregates linked incompatible experiences producing numerous divisions in mental apparatus. Current findings indicate that psychological splitting in schizophrenia is likely specifically presented on a neural level as disrupted organization in neural communication. This disrupted neural communication likely underlies deficits in mental processing described by various neuroscientific concepts such as theories of disturbed connectivity, corollary discharges and dynamic complexity. In this context, a purpose of the theoretical part of the dissertation is to describe basic neuroscience theories that complementarily reflect interrelated processes between mind and brain underlying disturbances of mental integration that likely present a neural representation of the splitting. A purpose of the first part of the empirical research was to examine relationships between psychological process of splitting and disturbed cognitive and affective functions in schizophrenia. A sample of patients with borderline personality disorder (BPD) was used as a control group in this study. Methods: In the clinical study, we have assessed 30 patients with schizophrenia and 35 patients with BPD. The symptoms of splitting were measured using self- reported...
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Burnout syndrome in Health Care Professionals.
Riethof, Norbert ; Bob, Petr (advisor) ; Světlák, Miroslav (referee) ; Pauknerová, Daniela (referee)
N. Riethof - Burnout syndrome in Health Care Professionals ABSTRACT Burnout syndrome is a state of total exhaustion related to work condi- tions and prolonged stress. While initial phases of burnout resemble stress symptoms, final phases of burnout are characterized by feelings of hopelessness, loss of meaning and desperation that have similar qual- ities as depression as well as existential vacuum described by Frankl in his logotherapy. In addition, the burnout syndrome involves stages in which people detach from their emotions and feelings as a defense mechanism against stress and have decreased ability to experience their own feelings and emotional states. Burnout usually begins with feelings of enthusiasm and idealized visualizations and it is in contrast with sub- sequent disillusionment, disappointment experienced later. After decades of burnout research, there is still a need for better def- inition of this condition including more precise diagnostic criteria and internationally recognized measurement tools, especially within health care system where the risks of unrecognized and untreated burnout are high. This study is focused on examination of potential causes of burn- out and relationships of burnout symptoms with certain personality traits, defense mechanisms and coping reactions including...
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Sport as Local Cleavage: Case Study of Vysočina Region
Kacetlová, Kateřina ; Jüptner, Petr (advisor) ; Bubeníček, Václav (referee)
The diploma thesis Sport as Local Cleavages: Case Study of Vysočina Region analyses whether sport and sport related topics (sport financing and other support, organization of sports events, operation and building of sport facilities, association activities, etc.) are able to divide a society, or whether it can be one of the cleavages at the local level in the Vysočina Region and what intensity this cleavage could have in the examined municipalities. The theoretical part contains the concept of conflict lines of S. Rokkan and S. M. Lipset. The research put emphasis on the local level of politics, the Czech environment and the cleavage connected with a sport. Authors such as S.Balík, V. Bubeníček, J. Čmejrek, J. Čopík, V. Hloušek and M. Kubálek are dealing with these issues. The analytical part of the thesis is a case study that examines the presence of cleavage, its intensity and the approach of actors through both semi-structured interviews with representatives of selected municipalities, records of municipalities and as well through media news monitoring. Based on research it is obvious that sport could evoke and develop conflicts and, in some cases, can indeed significantly divide a society and establish a new local policy player.
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Polish Politics and its Polarization after Smolensk
Harzer, Filip ; Koubek, Jiří (advisor) ; Doubek, Vratislav (referee)
This bachelor thesis analyzes influence of the Smolensk aircraft catastrophe on Polish politics. Work is based on the analysis of internal political development and changes in the political language of the relevant actors and it identifies sources of polarization of Polish politics after 2010. Theoretical framework is an attempt of Tomasz Zarycki to synthesise Rokkan's and Lipset's theory of cleavages with Kitschelt's concept of political conflict. The main part is an analysis of the language of political actors which includes selected events in the period between the catastrophy and the end of 2011. Key words Polarization, cleavages, centre, periphery, Smolensk, Poland, Civic Platform, Law and Justice, Kaczyński, Tusk
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Šumava National Park and Interests of its Municipalities: The Case of Postmaterial Cleavages?
Musilová, Karolína ; Jüptner, Petr (advisor) ; Švec, Kamil (referee)
The diploma thesis Šumava National Park and Interests of its Municipalities: The Case of Postmaterial Cleavages? analyses, if the postmaterial cleavage is present in the case of Šumava National Park and how is it projected on the national level. Since the very beginning, there have been argues about the notion of the national park, where two different set of ideas collide. On the one hand, there are local municipalities which depend on incomes from tourism, on the other hand, the environmentalists seek for growing extent of areas without any human intervention. The theoretical background is based on concept of cleavages by S. Rokkan and S.M. Lipset. Special attention is paid to the postmaterial dimension of the cleavage, which was evolved by R. Inglehart. The analytical part consists of case study, which examines the attitudes of the stakeholders. The examination is based on semi-structured interviews with mayors, official documents and media coverage of the topic. According to the findings, there is a cleavage, which is visible on all levels of political system, including political parties and presidents.
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Splitting and Dissociation in Schizophrenia
Pěč, Ondřej ; Raboch, Jiří (advisor) ; Šípek, Jiří (referee) ; Kocourková, Jana (referee)
The term splitting is defined as a process of formation of mental aggregates linked incompatible experiences producing numerous divisions in mental apparatus. Current findings indicate that psychological splitting in schizophrenia is likely specifically presented on a neural level as disrupted organization in neural communication. This disrupted neural communication likely underlies deficits in mental processing described by various neuroscientific concepts such as theories of disturbed connectivity, corollary discharges and dynamic complexity. In this context, a purpose of the theoretical part of the dissertation is to describe basic neuroscience theories that complementarily reflect interrelated processes between mind and brain underlying disturbances of mental integration that likely present a neural representation of the splitting. A purpose of the first part of the empirical research was to examine relationships between psychological process of splitting and disturbed cognitive and affective functions in schizophrenia. A sample of patients with borderline personality disorder (BPD) was used as a control group in this study. Methods: In the clinical study, we have assessed 30 patients with schizophrenia and 35 patients with BPD. The symptoms of splitting were measured using self- reported...
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